Application Note
EELS elemental mapping of cryogenically frozen mitochondria in human retinal ganglion cells
August 1, 2025
Calcium
Element density: 1.55 g/cm3
Electron configuration: [Ar]4s2
Oxidation states: +2
Edges
Explore electron energy loss spectroscopy (EELS) reference spectra, featuring major and minor edges used for elemental identification and chemical state analysis in materials research.
Major edges | |||
|---|---|---|---|
K | 4038 |
|  |
L2 | 350 |  |  |
L3 | 346 | | |
M2,3 | 25 |  | |
Minor edges | |||
|---|---|---|---|
| L1 | 438 | | |
| M1 | 44 |  | |
Considerations
L-edge white line resonance allow very high sensitivity and trace analysis.
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Related spectral data
CaCO3 thin film (0 – 920 eV)
Material analyzed: CaCO3
Related elements: Carbon, Calcium, Oxygen
Data contributor: Gatan
Beam energy (keV): 200
Nominal energy range (eV): 0 – 920
Collection semi-angle effective (mrad): 100
Notes: Serial EELS; from Ca evaporated onto NaCl; zero-loss peak intensity = 4.42e8; total elastic int. / zero-loss int. = 0.44; gain change factor = 513; 50 sweeps 20 ms dwell
Applications
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Application Note
Dose fractionation using multi-pass in-situ spectrum imaging
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The characterization of beam-induced phase changes with in-situ EELS
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