Application Note
EELS elemental mapping of cryogenically frozen mitochondria in human retinal ganglion cells
August 1, 2025
Nitrogen
Element density: 1.25 g/L
Electron configuration: [He]2s22p3
Oxidation states: +1,2,3,4,5/-1,2,3
Edges
Explore electron energy loss spectroscopy (EELS) reference spectra, featuring major and minor edges used for elemental identification and chemical state analysis in materials research.
Major edges | |||
|---|---|---|---|
K | 402 |
|  |
Considerations
The N K-edge shows rich fine structure and chemical shifts identifying local bonding effects. Nitrogen can be mobile under the beam and can show rapid change of concentration under a focused beam. Nitrogen in Si-oxynitride has been shown to pile up in front of a scanned beam.
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Related spectral data
Nitrogen, air at 8 torr (150 – 620 eV)
Material analyzed: N2
Related elements: Nitrogen
Data contributor: Gatan
Beam energy (keV): 100
Nominal energy range (eV): 150 – 620
Collection semi-angle effective (mrad): 4.1
Notes: Parallel EELS; no gain variation correction; n-K first peak at 400.2 eV (absolute volt. to drift tube)
Nitrogen, air at 8 torr (370 – 570 eV)
Material analyzed: N2
Related elements: Nitrogen
Data contributor: Gatan
Beam energy (keV): 100
Nominal energy range (eV): 370 – 570
Collection semi-angle effective (mrad): 4.1
Notes: Parallel EELS; no gain variation correction; n-K first peak at 400.2 eV (absolute volt. to drift tube)
BN particle suspended on holey C (0 – 910 eV)
Material analyzed: BN
Related elements: Boron, Nitrogen
Data contributor: Gatan
Beam energy (keV): 200
Nominal energy range (eV): 0 – 910
Collection semi-angle effective (mrad): 100
Notes: Serial EELS; zero-loss peak intensity = 1.81e8; total elastic int. / zero-loss int. = 0.77; gain change = 292; 50 sweeps 10 ms dwell
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